Fixation and fixatives in histopathology: a review

نویسندگان

چکیده

If fixation is not adequate, the other processes that follow such as dehydration, clearing, infiltration, embedding, microtomy and staining, will also be inadequate. A poorly processed tissue make it difficult for Pathologist/Histoscientist to render a proper diagnosis. This article aimed at elucidating more on fixatives process in histopathology. The information presented this review was gathered primarily from an extensive literature search PubMed, Scopus Textbooks. Fixatives histopathology can grouped into simple compound fixatives. Simple fixative solution or gas which contains only one active ingredient has singlechemical solution. Examples include; Formaldehyde, Glutaraldehyde, Mercuric Chloride, Potassium Dichromate, Picric Acid, Osmium Tetroxide, Acetic Ethanol, Acetone Chromic Acid. When two are combined solution, resulting called fixative. further divided Microanatomical cytological of micro-anatomical 10% formal-saline, Heidenhain’s Susa, Boiun’s fluid, Formol Sublimate. Cytological cytoplasmic nuclear Fixation terminates any ongoing biochemical reactions, increases mechanical strength stability treated tissues. To accomplish this, samples usually immersed immediately fluid. employed prevent autolysis putrefaction taking place. integral substances used forestall actions samples.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Assessment of fixatives, fixation, and tissue processing on morphology and RNA integrity.

Molecular characterization of morphologic change requires exquisite tissue morphology and RNA preservation; however, traditional fixatives usually result in fragmented RNA. To optimize molecular analyses on fixed tissues, we assessed morphologic and RNA integrity in rat liver when sections were fixed in 70% neutral-buffered formalin, modified Davidson's II, 70% ethanol, UMFIX, modified Carnoy's...

متن کامل

Comparison of fixatives and fixation time for PCR detection of Mycobacterium in zebrafish Danio rerio .

Mycobacteriosis is a common disease of laboratory zebrafish Danio rerio. Different infection patterns occur in zebrafish depending on mycobacterial species. Mycobacterium marinum and M. haemophilum produce virulent infections associated with high mortality, whereas M. chelonae is more widespread and is not associated with high mortality. Identification of mycobacterial infections to the species...

متن کامل

passivity in waiting for godot and endgame: a psychoanalytic reading

this study intends to investigate samuel beckett’s waiting for godot and endgame under the lacanian psychoanalysis. it begins by explaining the most important concepts of lacanian psychoanalysis. the beckettian characters are studied regarding their state of unconscious, and not the state of consciousness as is common in most beckett studies. according to lacan, language plays the sole role in ...

a contrastive analysis of concord and head parameter in english and azerbaijani

این پایان نامه به بررسی و مقایسه دو موضوع مطابقه میان فعل و فاعل (از نظر شخص و مشار) و هسته عبارت در دو زبان انگلیسی و آذربایجانی می پردازد. اول رابطه دستوری مطابقه مورد بررسی قرار می گیرد. مطابقه به این معناست که فعل مفرد به همراه فاعل مفرد و فعل جمع به همراه فاعل جمع می آید. در انگلیسی تمام افعال، بجز فعل بودن (to be) از نظر شمار با فاعلشان فقط در سوم شخص مفرد و در زمان حال مطابقت نشان میدهند...

15 صفحه اول

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Bayero Journal of Pure and Applied Sciences

سال: 2022

ISSN: ['2006-6996']

DOI: https://doi.org/10.4314/bajopas.v15i1.32